Protein Variants | Comment | Organism |
---|---|---|
additional information | three truncation mutants of the N-terminal fragment of the enzyme still activate the epithelial sodium channel | Homo sapiens |
S195A | site-directed mutagenesis, the active site mutant is not able to activate the epithelial sodium channel in contrast to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
plasminogen activator inhibitor 1 | PAI-1, complete inhibition, elevated inhibitor specifically inactivates pulmonary urokinase-type plasminogen activator and tissue.type plasminogen activator, EC 3.4.21.68 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | single-chain enzyme is converted to the two-chain form by incubation with immobilized plasmin, it can also be cleaved by plasmin at Lys158-Ile159 to produce potent two-chain enzyme accompanied by a conformational change in protein | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | enzyme level is extremely elevated in the edematous fluid of acutely injured lungs and pleurae | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epithelial sodium channel gamma subunit + H2O | of Xenopus laevis ocytes, activation by proteolytic cleavage at 177GR-/-KR180 | Homo sapiens | ? | - |
? | |
additional information | no activity with the epithelial sodium channel alpha subunit, the enzyme shows amidolytic activity with fluorogenic substrate Pefafluor uPA | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
uPA | - |
Homo sapiens |
Urokinase plasminogen activator | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | activation of alphabetagamma-epithelial sodium channel specifically by the enzyme may contribute to fluid clearance under physiological conditions and in injured tissues, multifaceted mechanisms for enzyme-mediated up-regulation of the epithelial sodium channel, which form the cellular and molecular rational for the beneficial effects of urokinase in mitigating mortal pulmonary edema and pleural effusions, overview | Homo sapiens |